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Neuron Specific Enolase

Rabbit Polyclonal Antibody

PRODUCT #: 40-1282

 

DESCRIPTION: Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway and the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
   
SPECIES CROSS-REACTIVITY: Human, mouse, rat

 

APPLICATIONS/DILUTIONS:        ICC/IF (1/200)
WB (1/1,000)
   
SOURCE: Rabbits were immunized with recombinant full length human NSE purified from E. coli.
   
FORM/STORAGE: 0.1 ml with 50% glycerol, 0.01% sodium azide. 
Store at -20º C.  Avoid multiple freeze/thaw cycles.
   
Reference: Shaw, G. et al. FASEB J. 16:869-871 (2002).
Begaz, T. et al. J. Neurotrauma 23:1201-1210 (2006).
   
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURE