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Neurofilament, NF-H (Phosphate Independent)

Rabbit Polyclonal Antibody

PRODUCT #: 40-1277

 

DESCRIPTION: Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant and biochemically stable. The form of NF-H expressed in neurons varies in terms of the level of phosphorylation. Mammalian NF-H contains tandem repeats based on the sequence lysine-serine-proline (KSP), which may be present up to 60 times, with some variability between species. These KSP sequences are the sites of phosphorylation, and in axons most if not all of the serine residues are phosphorylated. In contrast, neurofilaments in dendrites and perikarya are not phosphorylated on these KSP sequences. Axonal neurofilaments are therefore quite different immunologically from those in perikarya and dendrites, and many antibodies stain only axonal or only dendritic/perikaryal neurofilaments. Since bacteria do not phosphorylate mammalian proteins, this protein resembles the NF-H form characteristic of perikaryal and dendritic neurofilaments. Accordingly this antibody stains dendritic and perikaryal neurofilaments particularly well.
   
SPECIES CROSS-REACTIVITY: Human, mouse, rat

 

APPLICATIONS/DILUTIONS:        ICC/IF (1/200)
WB (1/2,000)
   
SOURCE: Rabbits were immunized with purifed NF-H/KSP region, expressed in E. coli.
   
FORM/STORAGE: 0.1 ml with 50% glycerol, 0.01% sodium azide. 
Store at -20º C.  Avoid multiple freeze/thaw cycles.
   
Reference: Harris, J., Ayyub, C. and Shaw G. J Neurosci Res 30:47-62 1991.
   

 
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.