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Microphthalmia Transcription Factor (Mi/MITF; Clone D5)

Mouse Monoclonal Antibody

PRODUCT #: 45-6803

 

DESCRIPTION: The Microphthalmia transcription factor (~61-64 kDa) is a transcription factor belonging to the basic helix-loop-helix leucine zipper (bHLH-ZIP) family of transcription factors that exists in a dimeric form.  Identified in several different species, the protein is called Mi in the mouse and MITF in man. Mi/MITF has several functions including regulation of the development and survival of many cell types.  While the primary function of Mi/MITF is transactivation of the genes involved in regulation of melanogenesis, it has also been shown to be required for osteoclast activity.  Mi/MITF exists as at least three different isoforms, Mitf-A/MITF-A, Mitf-H/MITF-H, Mitf-M/MITF-M that vary in tissue expression, as splice variants, and undergoes variable phosphorylation in a MAPK regulated fashion.  Mi/MITF has been shown to associate with several protein partners including CBP/p300, c-fos and PU-1 forming cytoplasmic complexes that are subsequently translocated to the nucleus.  The association with p300 is regulated in a phospho-specific manner making Mi/MITF one of only two proteins to be so regulated.  Mutations in MITF have been associated with Waardenburg-Syndrome Type 2.
 

SPECIES CROSS-REACTIVITY:

Human
Not mouse or rat.
 

APPLICATIONS/DILUTIONS:

FFPE (1.0-5.0 ug/ml)
IF (1.0-2.5 ug/ml)
IP (1.0 ug/sample)
WB (1.0 ug/ml)
 

SOURCE:

Balb/c mice were immunized with recombinant human Mi/MITF protein and fusing the splenocytes with P3-X63-Ag8-653 mouse myeloma cells.
 

FORM/STORAGE:

100 ug purified IgG1 with 50% glycerol, 0.01% sodium azide and 1.0 mg/ml BSA. Store at -20º C. Avoid multiple freeze/thaw cycles.

 

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS OR DIAGNOSTIC PROCEDURES.